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KMID : 0525720140190020087
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Journal of Chitin and Chitosan
2014 Volume.19 No. 2 p.87 ~ p.92
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Isolation and Characterization of a Bacterial Isolate for Polyhydroxyalkanoates Production
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Kim Kyung-Won
Park Jae-Kweon
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Abstract
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Microorganism accumulating polyhydroxyalkanoates (PHA) in its cytosol was isolated from water obtained from InCheon area. This strain was determined to be Bacillus species that was examined by 16S rDNA sequences and phylogenetic tree analysis. The bacteria accumulated up to 20.6% of PHA within 48 hours at 37oC in the presence of 2.0% glucose substrate under aerobic condition. The present work also investigates the influence of substrate on PHA production to find a clue trigger. The PHA accumulation increased to 23.5 and 23.3% containing 2.0% salicylic acid and lactose, respectively. In contrast both glucosamine and xylose have negative effect on PHA production for this bacterial strain. To the best of our knowledge, this report firstly demonstrates the effects of various sugars on PHA production.
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KEYWORD
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Polyhydroxyalkanoate, Bacillus, glucosamine, Salicylic acid, 16S rDNA sequences, Phylogenetic tree analysis
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